mouse anti human cd66a pe  (R&D Systems)

Journal: Molecular medicine reports

Article Title: Expansion of decidual CD45RO⁺ T cells with high expression of CEACAM1 in the early stage of pregnancy.

doi: 10.3892/mmr.2013.1552

Figure Lengend Snippet: Figure 2. Expression of CEACAM1 on the surface of T cells in first trimester human decidua and in the PB of healthy non-pregnant and pregnant females. (A) The percentages of CEACAM1+ cells among CD4+ T cells, CD8+ T cells, CD4+CD45RO+ T cells and CD8+CD45RO+ T cells are shown. The percentage of CD4+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) or pregnant females (P<0.001). The percentage of CD8+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P=0.004) or pregnant females (P=0.01). The percentage of CD4+CD45RO+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) and pregnant females (P=0.001). The percentage of CD8+CD45RO+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) and pregnant females (P<0.001). Data are presented as the mean ± SD. *P<0.05. (B) The isolated lymphocytes were analyzed for phenotypic frequency by flow cytometry. CD4+CD45RO+ and CD8+CD45RO+ T cells were individually gated and further analysis was conducted. Markers were set according to CEACAM1. PB, peripheral blood; CEACAM1, carcinoembryonic antigen-related cell adhesion molecule 1.

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD4 monoclonal antibody (mAb) (Jingmei Biological, Co., Beijing, China), CD8 mAb (Becton-Dickinson, Franklin Lakes, NJ, USA), phycoerythrin (PE)-conjugated mouse anti-human CEACAM1 mAb (R&D Systems, Minneapolis, MN, USA), PE-cyanine (Cy) 5-conjugated mouse anti-human CD45RO mAb (Jingmei Biological Co.) and their isotype- and fluorochrome-matched control antibodies, were used for flow cytometry.

Techniques: Expressing, Isolation, Flow Cytometry

Journal: Molecular medicine reports

Article Title: Expansion of decidual CD45RO⁺ T cells with high expression of CEACAM1 in the early stage of pregnancy.

doi: 10.3892/mmr.2013.1552

Figure Lengend Snippet: Figure 5. Expression of CEACAM1 on the surface of CD45RO+ T cells in the induced CD45RO+ T cell model. (A) The percentages of CD4+CD45RO+ and CD8+CD45RO+ T cells expressing CEACAM1 in the induced CD45RO+ T cell model are shown. The experiments were performed three times. The propor tion of CEACAM1-expressing CD4+CD45RO+ T cells was significantly increased in the group with conditioned medium from the coculture of monocytes and the human trophoblast HTR8/SVneo cell line (the MHM group) compared with the control group (P=0.02). The proportion of CEACAM1-expressing CD4+CD45RO+ T cells was not significantly decreased in the MHM + anti‑MCP‑1 group, compared with that in the MHM group; however, that proportion was significantly increased by human recombinant MCP‑1 (rhMCP‑1) compared with the control group (P=0.017). The proportion of CEACAM1-expressing CD8+CD45RO+ T cells did not show a statistically significant difference among the four groups (P=0.303). Data are presented as the mean ± SD. *P<0.05. (B) The lymphocytes were analyzed for phenotypic frequency by flow cytometry. CD4+CD45RO+ and CD8+CD45RO+ T cells were individually gated and further analysis was conducted. Markers were set according to the CEACAM1‑isotype IgG. CEACAM1, carcinoembryonic antigen-related cell adhesion molecule 1; MCP-1, monocyte chemoattractant protein-1.

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD4 monoclonal antibody (mAb) (Jingmei Biological, Co., Beijing, China), CD8 mAb (Becton-Dickinson, Franklin Lakes, NJ, USA), phycoerythrin (PE)-conjugated mouse anti-human CEACAM1 mAb (R&D Systems, Minneapolis, MN, USA), PE-cyanine (Cy) 5-conjugated mouse anti-human CD45RO mAb (Jingmei Biological Co.) and their isotype- and fluorochrome-matched control antibodies, were used for flow cytometry.

Techniques: Expressing, Control, Recombinant, Flow Cytometry