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mouse anti human cd66a pe  (R&D Systems)


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    R&D Systems mouse anti human cd66a pe
    Sequencing data reveal differentially expressed miRNAs in colonic epithelial cells of patients with active and quiescent UC. [A] FACS of colonic epithelial cells and distribution of crypt-top CD44 − <t>CD66a</t> + and crypt-bottom CD44 + CD66a − epithelial cell types in inflammatory [aUC] [ n = 16] and non-inflammatory [qUC and HC] [ n = 15 and 17] colon tissues. Each dot represents a sample from the patients of the second study group. Mean ± SD of each group is represented by vertical lines. To compare the groups a non-parametric Mann–Whitney U test was performed, * p < 0.05. [B] MDS plot showing the similarity structure of the miRNA transcriptomes in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in active UC [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and HC [ n = 17] based on normalized expression values. Dots represent samples shaped by cell population. Dot colours represent condition. The centroid of ellipses corresponds to the condition group mean; the shapes are defined by covariance within the group. [C, D] Volcano plots of differentially expressed miRNAs in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in aUC [ n = 16], qUC [ n = 15], and HC [ n = 17]. Colours indicate significantly [FDR < 0.05] differentially expressed miRNAs with an absolute value of log 2 FC > 1 between compared groups. [E–G] Venn diagrams representing the numbers of commonly and uniquely differentially expressed miRNAs in [E] crypt-bottom [CD44 + ] and [F] crypt-top [CD66a + ] epithelial cell populations in different UC activity, and [G] between crypt-bottom and crypt-top cells in the same condition. [H] Overrepresented pathways with the top five FDR values between crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations during aUC [ n = 16], qUC [ n = 15], and HC [ n = 17] identified by miRNA–target gene set enrichment analysis. Dot size represents the number of miRNA gene–target counts in the significantly enriched [FDR < 0.05] GO categories.
    Mouse Anti Human Cd66a Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human cd66a pe/product/R&D Systems
    Average 93 stars, based on 19 article reviews
    mouse anti human cd66a pe - by Bioz Stars, 2026-03
    93/100 stars

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    1) Product Images from "The microRNA Expression in Crypt-Top and Crypt-Bottom Colonic Epithelial Cell Populations Demonstrates Cell-Type Specificity and Correlates with Endoscopic Activity in Ulcerative Colitis"

    Article Title: The microRNA Expression in Crypt-Top and Crypt-Bottom Colonic Epithelial Cell Populations Demonstrates Cell-Type Specificity and Correlates with Endoscopic Activity in Ulcerative Colitis

    Journal: Journal of Crohn's & Colitis

    doi: 10.1093/ecco-jcc/jjae108

    Sequencing data reveal differentially expressed miRNAs in colonic epithelial cells of patients with active and quiescent UC. [A] FACS of colonic epithelial cells and distribution of crypt-top CD44 − CD66a + and crypt-bottom CD44 + CD66a − epithelial cell types in inflammatory [aUC] [ n = 16] and non-inflammatory [qUC and HC] [ n = 15 and 17] colon tissues. Each dot represents a sample from the patients of the second study group. Mean ± SD of each group is represented by vertical lines. To compare the groups a non-parametric Mann–Whitney U test was performed, * p < 0.05. [B] MDS plot showing the similarity structure of the miRNA transcriptomes in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in active UC [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and HC [ n = 17] based on normalized expression values. Dots represent samples shaped by cell population. Dot colours represent condition. The centroid of ellipses corresponds to the condition group mean; the shapes are defined by covariance within the group. [C, D] Volcano plots of differentially expressed miRNAs in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in aUC [ n = 16], qUC [ n = 15], and HC [ n = 17]. Colours indicate significantly [FDR < 0.05] differentially expressed miRNAs with an absolute value of log 2 FC > 1 between compared groups. [E–G] Venn diagrams representing the numbers of commonly and uniquely differentially expressed miRNAs in [E] crypt-bottom [CD44 + ] and [F] crypt-top [CD66a + ] epithelial cell populations in different UC activity, and [G] between crypt-bottom and crypt-top cells in the same condition. [H] Overrepresented pathways with the top five FDR values between crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations during aUC [ n = 16], qUC [ n = 15], and HC [ n = 17] identified by miRNA–target gene set enrichment analysis. Dot size represents the number of miRNA gene–target counts in the significantly enriched [FDR < 0.05] GO categories.
    Figure Legend Snippet: Sequencing data reveal differentially expressed miRNAs in colonic epithelial cells of patients with active and quiescent UC. [A] FACS of colonic epithelial cells and distribution of crypt-top CD44 − CD66a + and crypt-bottom CD44 + CD66a − epithelial cell types in inflammatory [aUC] [ n = 16] and non-inflammatory [qUC and HC] [ n = 15 and 17] colon tissues. Each dot represents a sample from the patients of the second study group. Mean ± SD of each group is represented by vertical lines. To compare the groups a non-parametric Mann–Whitney U test was performed, * p < 0.05. [B] MDS plot showing the similarity structure of the miRNA transcriptomes in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in active UC [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and HC [ n = 17] based on normalized expression values. Dots represent samples shaped by cell population. Dot colours represent condition. The centroid of ellipses corresponds to the condition group mean; the shapes are defined by covariance within the group. [C, D] Volcano plots of differentially expressed miRNAs in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in aUC [ n = 16], qUC [ n = 15], and HC [ n = 17]. Colours indicate significantly [FDR < 0.05] differentially expressed miRNAs with an absolute value of log 2 FC > 1 between compared groups. [E–G] Venn diagrams representing the numbers of commonly and uniquely differentially expressed miRNAs in [E] crypt-bottom [CD44 + ] and [F] crypt-top [CD66a + ] epithelial cell populations in different UC activity, and [G] between crypt-bottom and crypt-top cells in the same condition. [H] Overrepresented pathways with the top five FDR values between crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations during aUC [ n = 16], qUC [ n = 15], and HC [ n = 17] identified by miRNA–target gene set enrichment analysis. Dot size represents the number of miRNA gene–target counts in the significantly enriched [FDR < 0.05] GO categories.

    Techniques Used: Sequencing, MANN-WHITNEY, Expressing, Activity Assay

    WGCNA in colonic epithelial cell populations reveals miRNA co-expression modules which reflect endoscopic activity of UC. [A] Network displaying identified co-expression modules [M1 and M2] in crypt-top [CD66a] and crypt-bottom [CD44] colonic epithelial cells of patients with active [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and control individuals [ n = 17]. The colour and size of the node represents distinct modules and strength of connectivity, respectively. [B] Dot plot showing normalized enrichment score [NES] of modules M1 and M2 in crypt-top [CD66a] and crypt-bottom [CD44] colonic epithelial cells of patients with active UC, quiescent UC, and control individuals. The colour and size of the dot represents the value and absolute value of NES, respectively. The box marks significant value [ p adj, < 0.05]. Plots [C] and [E] show the correlation between module M1 eigengene value and Mayo endoscopic activity in crypt-bottom [CD44] and crypt-top [CD66a] colonic epithelial cells as well as colon tissue, respectively. rho—Spearman correlation coefficient. Each dot represents a different sample. Plots [D] and [F] show the AUC-ROC curves reflecting the performance of M1 module eigengene value at distinguishing between active UC [aUC] and quiescent UC [qUC] in crypt-bottom [CD44] and crypt-top [CD66a] colonic epithelial cells as well as in colon tissue, respectively.
    Figure Legend Snippet: WGCNA in colonic epithelial cell populations reveals miRNA co-expression modules which reflect endoscopic activity of UC. [A] Network displaying identified co-expression modules [M1 and M2] in crypt-top [CD66a] and crypt-bottom [CD44] colonic epithelial cells of patients with active [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and control individuals [ n = 17]. The colour and size of the node represents distinct modules and strength of connectivity, respectively. [B] Dot plot showing normalized enrichment score [NES] of modules M1 and M2 in crypt-top [CD66a] and crypt-bottom [CD44] colonic epithelial cells of patients with active UC, quiescent UC, and control individuals. The colour and size of the dot represents the value and absolute value of NES, respectively. The box marks significant value [ p adj, < 0.05]. Plots [C] and [E] show the correlation between module M1 eigengene value and Mayo endoscopic activity in crypt-bottom [CD44] and crypt-top [CD66a] colonic epithelial cells as well as colon tissue, respectively. rho—Spearman correlation coefficient. Each dot represents a different sample. Plots [D] and [F] show the AUC-ROC curves reflecting the performance of M1 module eigengene value at distinguishing between active UC [aUC] and quiescent UC [qUC] in crypt-bottom [CD44] and crypt-top [CD66a] colonic epithelial cells as well as in colon tissue, respectively.

    Techniques Used: Expressing, Activity Assay, Control



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    Image Search Results


    Sequencing data reveal differentially expressed miRNAs in colonic epithelial cells of patients with active and quiescent UC. [A] FACS of colonic epithelial cells and distribution of crypt-top CD44 − CD66a + and crypt-bottom CD44 + CD66a − epithelial cell types in inflammatory [aUC] [ n = 16] and non-inflammatory [qUC and HC] [ n = 15 and 17] colon tissues. Each dot represents a sample from the patients of the second study group. Mean ± SD of each group is represented by vertical lines. To compare the groups a non-parametric Mann–Whitney U test was performed, * p < 0.05. [B] MDS plot showing the similarity structure of the miRNA transcriptomes in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in active UC [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and HC [ n = 17] based on normalized expression values. Dots represent samples shaped by cell population. Dot colours represent condition. The centroid of ellipses corresponds to the condition group mean; the shapes are defined by covariance within the group. [C, D] Volcano plots of differentially expressed miRNAs in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in aUC [ n = 16], qUC [ n = 15], and HC [ n = 17]. Colours indicate significantly [FDR < 0.05] differentially expressed miRNAs with an absolute value of log 2 FC > 1 between compared groups. [E–G] Venn diagrams representing the numbers of commonly and uniquely differentially expressed miRNAs in [E] crypt-bottom [CD44 + ] and [F] crypt-top [CD66a + ] epithelial cell populations in different UC activity, and [G] between crypt-bottom and crypt-top cells in the same condition. [H] Overrepresented pathways with the top five FDR values between crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations during aUC [ n = 16], qUC [ n = 15], and HC [ n = 17] identified by miRNA–target gene set enrichment analysis. Dot size represents the number of miRNA gene–target counts in the significantly enriched [FDR < 0.05] GO categories.

    Journal: Journal of Crohn's & Colitis

    Article Title: The microRNA Expression in Crypt-Top and Crypt-Bottom Colonic Epithelial Cell Populations Demonstrates Cell-Type Specificity and Correlates with Endoscopic Activity in Ulcerative Colitis

    doi: 10.1093/ecco-jcc/jjae108

    Figure Lengend Snippet: Sequencing data reveal differentially expressed miRNAs in colonic epithelial cells of patients with active and quiescent UC. [A] FACS of colonic epithelial cells and distribution of crypt-top CD44 − CD66a + and crypt-bottom CD44 + CD66a − epithelial cell types in inflammatory [aUC] [ n = 16] and non-inflammatory [qUC and HC] [ n = 15 and 17] colon tissues. Each dot represents a sample from the patients of the second study group. Mean ± SD of each group is represented by vertical lines. To compare the groups a non-parametric Mann–Whitney U test was performed, * p < 0.05. [B] MDS plot showing the similarity structure of the miRNA transcriptomes in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in active UC [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and HC [ n = 17] based on normalized expression values. Dots represent samples shaped by cell population. Dot colours represent condition. The centroid of ellipses corresponds to the condition group mean; the shapes are defined by covariance within the group. [C, D] Volcano plots of differentially expressed miRNAs in crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations in aUC [ n = 16], qUC [ n = 15], and HC [ n = 17]. Colours indicate significantly [FDR < 0.05] differentially expressed miRNAs with an absolute value of log 2 FC > 1 between compared groups. [E–G] Venn diagrams representing the numbers of commonly and uniquely differentially expressed miRNAs in [E] crypt-bottom [CD44 + ] and [F] crypt-top [CD66a + ] epithelial cell populations in different UC activity, and [G] between crypt-bottom and crypt-top cells in the same condition. [H] Overrepresented pathways with the top five FDR values between crypt-top [CD66a + ] and crypt-bottom [CD44 + ] colonic epithelial cell populations during aUC [ n = 16], qUC [ n = 15], and HC [ n = 17] identified by miRNA–target gene set enrichment analysis. Dot size represents the number of miRNA gene–target counts in the significantly enriched [FDR < 0.05] GO categories.

    Article Snippet: Antibodies used in this study were selected based on previous study and included: mouse anti-human CD326/EpCAM-FITC [clone VU-1D9, Life Technologies], mouse anti-human CD44-APC [clone G44-26, BD Biosciences], and mouse anti-human CD66a-PE [clone 283340, R&D Systems].

    Techniques: Sequencing, MANN-WHITNEY, Expressing, Activity Assay

    WGCNA in colonic epithelial cell populations reveals miRNA co-expression modules which reflect endoscopic activity of UC. [A] Network displaying identified co-expression modules [M1 and M2] in crypt-top [CD66a] and crypt-bottom [CD44] colonic epithelial cells of patients with active [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and control individuals [ n = 17]. The colour and size of the node represents distinct modules and strength of connectivity, respectively. [B] Dot plot showing normalized enrichment score [NES] of modules M1 and M2 in crypt-top [CD66a] and crypt-bottom [CD44] colonic epithelial cells of patients with active UC, quiescent UC, and control individuals. The colour and size of the dot represents the value and absolute value of NES, respectively. The box marks significant value [ p adj, < 0.05]. Plots [C] and [E] show the correlation between module M1 eigengene value and Mayo endoscopic activity in crypt-bottom [CD44] and crypt-top [CD66a] colonic epithelial cells as well as colon tissue, respectively. rho—Spearman correlation coefficient. Each dot represents a different sample. Plots [D] and [F] show the AUC-ROC curves reflecting the performance of M1 module eigengene value at distinguishing between active UC [aUC] and quiescent UC [qUC] in crypt-bottom [CD44] and crypt-top [CD66a] colonic epithelial cells as well as in colon tissue, respectively.

    Journal: Journal of Crohn's & Colitis

    Article Title: The microRNA Expression in Crypt-Top and Crypt-Bottom Colonic Epithelial Cell Populations Demonstrates Cell-Type Specificity and Correlates with Endoscopic Activity in Ulcerative Colitis

    doi: 10.1093/ecco-jcc/jjae108

    Figure Lengend Snippet: WGCNA in colonic epithelial cell populations reveals miRNA co-expression modules which reflect endoscopic activity of UC. [A] Network displaying identified co-expression modules [M1 and M2] in crypt-top [CD66a] and crypt-bottom [CD44] colonic epithelial cells of patients with active [aUC] [ n = 16], quiescent UC [qUC] [ n = 15], and control individuals [ n = 17]. The colour and size of the node represents distinct modules and strength of connectivity, respectively. [B] Dot plot showing normalized enrichment score [NES] of modules M1 and M2 in crypt-top [CD66a] and crypt-bottom [CD44] colonic epithelial cells of patients with active UC, quiescent UC, and control individuals. The colour and size of the dot represents the value and absolute value of NES, respectively. The box marks significant value [ p adj, < 0.05]. Plots [C] and [E] show the correlation between module M1 eigengene value and Mayo endoscopic activity in crypt-bottom [CD44] and crypt-top [CD66a] colonic epithelial cells as well as colon tissue, respectively. rho—Spearman correlation coefficient. Each dot represents a different sample. Plots [D] and [F] show the AUC-ROC curves reflecting the performance of M1 module eigengene value at distinguishing between active UC [aUC] and quiescent UC [qUC] in crypt-bottom [CD44] and crypt-top [CD66a] colonic epithelial cells as well as in colon tissue, respectively.

    Article Snippet: Antibodies used in this study were selected based on previous study and included: mouse anti-human CD326/EpCAM-FITC [clone VU-1D9, Life Technologies], mouse anti-human CD44-APC [clone G44-26, BD Biosciences], and mouse anti-human CD66a-PE [clone 283340, R&D Systems].

    Techniques: Expressing, Activity Assay, Control

    Figure 2. Expression of CEACAM1 on the surface of T cells in first trimester human decidua and in the PB of healthy non-pregnant and pregnant females. (A) The percentages of CEACAM1+ cells among CD4+ T cells, CD8+ T cells, CD4+CD45RO+ T cells and CD8+CD45RO+ T cells are shown. The percentage of CD4+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) or pregnant females (P<0.001). The percentage of CD8+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P=0.004) or pregnant females (P=0.01). The percentage of CD4+CD45RO+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) and pregnant females (P=0.001). The percentage of CD8+CD45RO+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) and pregnant females (P<0.001). Data are presented as the mean ± SD. *P<0.05. (B) The isolated lymphocytes were analyzed for phenotypic frequency by flow cytometry. CD4+CD45RO+ and CD8+CD45RO+ T cells were individually gated and further analysis was conducted. Markers were set according to CEACAM1. PB, peripheral blood; CEACAM1, carcinoembryonic antigen-related cell adhesion molecule 1.

    Journal: Molecular medicine reports

    Article Title: Expansion of decidual CD45RO⁺ T cells with high expression of CEACAM1 in the early stage of pregnancy.

    doi: 10.3892/mmr.2013.1552

    Figure Lengend Snippet: Figure 2. Expression of CEACAM1 on the surface of T cells in first trimester human decidua and in the PB of healthy non-pregnant and pregnant females. (A) The percentages of CEACAM1+ cells among CD4+ T cells, CD8+ T cells, CD4+CD45RO+ T cells and CD8+CD45RO+ T cells are shown. The percentage of CD4+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) or pregnant females (P<0.001). The percentage of CD8+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P=0.004) or pregnant females (P=0.01). The percentage of CD4+CD45RO+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) and pregnant females (P=0.001). The percentage of CD8+CD45RO+CEACAM1+ T cells in the decidua was significantly increased compared with that in the PB from non-pregnant females (P<0.001) and pregnant females (P<0.001). Data are presented as the mean ± SD. *P<0.05. (B) The isolated lymphocytes were analyzed for phenotypic frequency by flow cytometry. CD4+CD45RO+ and CD8+CD45RO+ T cells were individually gated and further analysis was conducted. Markers were set according to CEACAM1. PB, peripheral blood; CEACAM1, carcinoembryonic antigen-related cell adhesion molecule 1.

    Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD4 monoclonal antibody (mAb) (Jingmei Biological, Co., Beijing, China), CD8 mAb (Becton-Dickinson, Franklin Lakes, NJ, USA), phycoerythrin (PE)-conjugated mouse anti-human CEACAM1 mAb (R&D Systems, Minneapolis, MN, USA), PE-cyanine (Cy) 5-conjugated mouse anti-human CD45RO mAb (Jingmei Biological Co.) and their isotype- and fluorochrome-matched control antibodies, were used for flow cytometry.

    Techniques: Expressing, Isolation, Flow Cytometry

    Figure 5. Expression of CEACAM1 on the surface of CD45RO+ T cells in the induced CD45RO+ T cell model. (A) The percentages of CD4+CD45RO+ and CD8+CD45RO+ T cells expressing CEACAM1 in the induced CD45RO+ T cell model are shown. The experiments were performed three times. The propor tion of CEACAM1-expressing CD4+CD45RO+ T cells was significantly increased in the group with conditioned medium from the coculture of monocytes and the human trophoblast HTR8/SVneo cell line (the MHM group) compared with the control group (P=0.02). The proportion of CEACAM1-expressing CD4+CD45RO+ T cells was not significantly decreased in the MHM + anti‑MCP‑1 group, compared with that in the MHM group; however, that proportion was significantly increased by human recombinant MCP‑1 (rhMCP‑1) compared with the control group (P=0.017). The proportion of CEACAM1-expressing CD8+CD45RO+ T cells did not show a statistically significant difference among the four groups (P=0.303). Data are presented as the mean ± SD. *P<0.05. (B) The lymphocytes were analyzed for phenotypic frequency by flow cytometry. CD4+CD45RO+ and CD8+CD45RO+ T cells were individually gated and further analysis was conducted. Markers were set according to the CEACAM1‑isotype IgG. CEACAM1, carcinoembryonic antigen-related cell adhesion molecule 1; MCP-1, monocyte chemoattractant protein-1.

    Journal: Molecular medicine reports

    Article Title: Expansion of decidual CD45RO⁺ T cells with high expression of CEACAM1 in the early stage of pregnancy.

    doi: 10.3892/mmr.2013.1552

    Figure Lengend Snippet: Figure 5. Expression of CEACAM1 on the surface of CD45RO+ T cells in the induced CD45RO+ T cell model. (A) The percentages of CD4+CD45RO+ and CD8+CD45RO+ T cells expressing CEACAM1 in the induced CD45RO+ T cell model are shown. The experiments were performed three times. The propor tion of CEACAM1-expressing CD4+CD45RO+ T cells was significantly increased in the group with conditioned medium from the coculture of monocytes and the human trophoblast HTR8/SVneo cell line (the MHM group) compared with the control group (P=0.02). The proportion of CEACAM1-expressing CD4+CD45RO+ T cells was not significantly decreased in the MHM + anti‑MCP‑1 group, compared with that in the MHM group; however, that proportion was significantly increased by human recombinant MCP‑1 (rhMCP‑1) compared with the control group (P=0.017). The proportion of CEACAM1-expressing CD8+CD45RO+ T cells did not show a statistically significant difference among the four groups (P=0.303). Data are presented as the mean ± SD. *P<0.05. (B) The lymphocytes were analyzed for phenotypic frequency by flow cytometry. CD4+CD45RO+ and CD8+CD45RO+ T cells were individually gated and further analysis was conducted. Markers were set according to the CEACAM1‑isotype IgG. CEACAM1, carcinoembryonic antigen-related cell adhesion molecule 1; MCP-1, monocyte chemoattractant protein-1.

    Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD4 monoclonal antibody (mAb) (Jingmei Biological, Co., Beijing, China), CD8 mAb (Becton-Dickinson, Franklin Lakes, NJ, USA), phycoerythrin (PE)-conjugated mouse anti-human CEACAM1 mAb (R&D Systems, Minneapolis, MN, USA), PE-cyanine (Cy) 5-conjugated mouse anti-human CD45RO mAb (Jingmei Biological Co.) and their isotype- and fluorochrome-matched control antibodies, were used for flow cytometry.

    Techniques: Expressing, Control, Recombinant, Flow Cytometry